One option is to use an SQL statement but WordPress provides a nice function called count_users.

When you call this function it will output a result like this.

Result

[code]
array (
'total_users' => 6,
'avail_roles' =>
array (
'administrator' => 2,
'editor' => 2,
'subscriber' => 2,
),
)
[/code]

Here is how to use it.

[code]
$result = count_users();
$total_cnt = empty( $result[ 'total_users' ] ) ? 0 : $result[ 'total_users' ];

echo 'Count: ' . $total_cnt;
[/code]

Do you use test/staging sites? Visit qSandbox for more info.

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